{"id":9480,"date":"2021-01-05T23:20:54","date_gmt":"2021-01-06T04:20:54","guid":{"rendered":"https:\/\/nebula.org\/blog\/methodes-de-test-dintroduction-a-ladn\/"},"modified":"2021-02-20T20:47:51","modified_gmt":"2021-02-21T01:47:51","slug":"methodes-de-test-dintroduction-a-ladn","status":"publish","type":"post","link":"https:\/\/nebula.org\/blog\/fr\/methodes-de-test-dintroduction-a-ladn\/","title":{"rendered":"Introduction aux m\u00e9thodes de test ADN"},"content":{"rendered":"\n

Edit\u00e9 par Christina Swords, Ph.D.<\/em><\/p>\n\n

Les tests g\u00e9n\u00e9tiques n’ont jamais \u00e9t\u00e9 aussi faciles. Les services de test ADN \u00e0 domicile continuent de gagner en popularit\u00e9 en tant que moyens d’en savoir plus sur votre ascendance et vos traits h\u00e9rit\u00e9s. Cependant, passer au crible toutes les entreprises et tous les types de tests ADN pour trouver le meilleur test ADN peut \u00eatre accablant. Ici, nous vous donnons un bref aper\u00e7u des diff\u00e9rents types de tests ADN. <\/p>\n\n

Technologies de test ADN<\/strong><\/h2>\n\n

Les puces \u00e0 ADN et le s\u00e9quen\u00e7age de nouvelle g\u00e9n\u00e9ration (NGS) sont deux technologies diff\u00e9rentes couramment utilis\u00e9es pour les tests g\u00e9n\u00e9tiques. Les deux peuvent \u00eatre utilis\u00e9s pour lire des informations g\u00e9n\u00e9tiques \u00e0 partir d’ADN chromosomique et mitochondrial. Cependant, les puces \u00e0 ADN et les technologies NGS fonctionnent tr\u00e8s diff\u00e9remment.<\/p>\n\n

G\u00e9notypage bas\u00e9 sur des puces \u00e0 ADN<\/h3>\n\n

Les puces \u00e0 ADN lisent l’ADN \u00e0 un ensemble d\u00e9fini de positions connues pour varier d’une personne \u00e0 l’autre. Ces positions sont appel\u00e9es polymorphismes nucl\u00e9otidiques simples (SNP). La plupart des services de tests g\u00e9n\u00e9tiques, tels que 23andMe et AncestryDNA, utilisent la technologie des puces \u00e0 ADN pour profiler (g\u00e9notyper) votre g\u00e9nome \u00e0 environ 500 000 positions. Cela repr\u00e9sente moins de 0,1% de l’ensemble du g\u00e9nome humain. Bien que le g\u00e9notypage \u00e0 base de micror\u00e9seaux soit tr\u00e8s abordable, il manque beaucoup d’informations importantes.<\/p>\n\n

S\u00e9quen\u00e7age de nouvelle g\u00e9n\u00e9ration<\/strong><\/h3>\n\n

Le s\u00e9quen\u00e7age de nouvelle g\u00e9n\u00e9ration (NGS) est une technologie qui permet de lire tr\u00e8s efficacement de grandes quantit\u00e9s d’informations g\u00e9n\u00e9tiques. NGS fonctionne en lisant de nombreux petits segments d’ADN, puis en les reconstituant pour d\u00e9terminer une s\u00e9quence continue (Figure 1). Contrairement aux puces \u00e0 ADN, NGS peut \u00eatre utilis\u00e9 pour lire des g\u00e9nomes entiers au lieu d’un petit nombre de positions. Cependant, alors que NGS produit beaucoup plus de donn\u00e9es que le g\u00e9notypage bas\u00e9 sur des micror\u00e9seaux, il a tendance \u00e0 \u00eatre plus co\u00fbteux. Il existe plusieurs tests ADN bas\u00e9s sur le NGS qui ont des prix diff\u00e9rents.<\/p>\n\n

\"Séquençage<\/figure>\n\n

Figure 1. S\u00e9quen\u00e7age de nouvelle g\u00e9n\u00e9ration (NGS). Image gracieuset\u00e9 de l’Institut national de recherche sur le g\u00e9nome humain. <\/p>\n\n

Tests ADN bas\u00e9s sur le NGS<\/strong><\/h2>\n\n

S\u00e9quen\u00e7age complet de l’exome (WES)<\/strong><\/h3>\n\n

Whole Exome Sequencing (WES) lit les ~ 1,5% du g\u00e9nome humain qui codent pour des prot\u00e9ines – des machines mol\u00e9culaires qui remplissent la plupart des fonctions cellulaires. Parce que WES ne couvre qu’une petite partie du g\u00e9nome humain, il est moins cher que WGS. Cependant, WES manque des variantes g\u00e9n\u00e9tiques qui sont en dehors des r\u00e9gions codant pour les prot\u00e9ines et ont souvent des fonctions de r\u00e9gulation importantes. En outre, le s\u00e9quen\u00e7age de l’exome entier est encore assez co\u00fbteux par rapport au g\u00e9notypage bas\u00e9 sur des micror\u00e9seaux. Ainsi, notre r\u00e9ponse \u00e0 la question de savoir s’il faut choisir le s\u00e9quen\u00e7age du g\u00e9nome entier ou le s\u00e9quen\u00e7age de l’exome entier est d’aller avec le s\u00e9quen\u00e7age du g\u00e9nome entier.<\/p>\n\n

S\u00e9quen\u00e7age du g\u00e9nome entier (WGS)<\/strong><\/h3>\n\n

La technologie NGS peut \u00eatre utilis\u00e9e pour lire un g\u00e9nome humain entier. Ceci est appel\u00e9 s\u00e9quen\u00e7age du g\u00e9nome entier (WGS). Il permet une d\u00e9couverte impartiale et compl\u00e8te des variantes g\u00e9n\u00e9tiques et donne un aper\u00e7u plus approfondi de la constitution g\u00e9n\u00e9tique personnelle. En plus de la lecture du g\u00e9nome entier, chaque position est souvent lue plusieurs fois afin d’augmenter la pr\u00e9cision des r\u00e9sultats. Par exemple, WGS est g\u00e9n\u00e9ralement effectu\u00e9 \u00e0 une couverture 30x (profondeur), ce qui signifie qu’en moyenne chaque position dans le g\u00e9nome est lue 30 fois. Cela rend le WGS beaucoup plus cher que le g\u00e9notypage bas\u00e9 sur des micror\u00e9seaux. <\/p>\n\n

G\u00e9nomique des n\u00e9buleuses<\/strong><\/h2>\n\n

Notre mission chez Nebula Genomics est de rendre le s\u00e9quen\u00e7age personnel du g\u00e9nome abordable pour tous. En f\u00e9vrier 2020, nous avons franchi une \u00e9tape importante en ramenant le co\u00fbt de S\u00e9quen\u00e7age du g\u00e9nome entier 30x en dessous de 300 $<\/a> . Cela rapproche le co\u00fbt du s\u00e9quen\u00e7age personnel du g\u00e9nome du co\u00fbt de tests g\u00e9n\u00e9tiques beaucoup moins complets. <\/p>\n\n

Voulez-vous en savoir plus sur la mani\u00e8re dont les technologies de test ADN sont appliqu\u00e9es? Jetez un \u0153il \u00e0 notre introduction aux tests de paternit\u00e9<\/a> !<\/p>\n","protected":false},"excerpt":{"rendered":"

Edit\u00e9 par Christina Swords, Ph.D. Les tests g\u00e9n\u00e9tiques n’ont jamais \u00e9t\u00e9 aussi faciles. Les services de test ADN \u00e0 domicile continuent de gagner en popularit\u00e9 en tant que moyens d’en savoir plus sur votre ascendance et vos traits h\u00e9rit\u00e9s. Cependant, …<\/p>\n

Introduction aux m\u00e9thodes de test ADN<\/span> Lire la suite \u00bb<\/a><\/p>\n","protected":false},"author":18,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"site-sidebar-layout":"default","site-content-layout":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","_FSMCFIC_featured_image_caption":"","_FSMCFIC_featured_image_nocaption":"","_FSMCFIC_featured_image_hide":"","footnotes":""},"categories":[4976],"tags":[],"class_list":["post-9480","post","type-post","status-publish","format-standard","hentry","category-science-fr"],"yoast_head":"\nIntroduction aux m\u00e9thodes de test ADN - Nebula Genomics Blog<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nebula.org\/blog\/fr\/methodes-de-test-dintroduction-a-ladn\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Introduction aux m\u00e9thodes de test ADN - Nebula Genomics Blog\" \/>\n<meta property=\"og:description\" content=\"Edit\u00e9 par Christina Swords, Ph.D. Les tests g\u00e9n\u00e9tiques n’ont jamais \u00e9t\u00e9 aussi faciles. 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