{"id":9558,"date":"2021-01-05T23:22:56","date_gmt":"2021-01-06T04:22:56","guid":{"rendered":"https:\/\/nebula.org\/blog\/dna-%e3%83%9d%e3%83%aa%e3%83%a1%e3%83%a9%e3%83%bc%e3%82%bc\/"},"modified":"2021-02-20T20:23:23","modified_gmt":"2021-02-21T01:23:23","slug":"dna-%e3%83%9d%e3%83%aa%e3%83%a1%e3%83%a9%e3%83%bc%e3%82%bc","status":"publish","type":"post","link":"https:\/\/nebula.org\/blog\/ja\/dna-%e3%83%9d%e3%83%aa%e3%83%a1%e3%83%a9%e3%83%bc%e3%82%bc\/","title":{"rendered":"DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc-\u30b2\u30ce\u30e0\u306e\u5f37\u529b\u306a\u4f5c\u5bb6"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\" id=\"h-dna-polymerase-definition\">DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u5b9a\u7fa9<\/h2>\n\n<p>DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u30c7\u30aa\u30ad\u30b7\u30ea\u30dc\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u304b\u3089\u306eDNA\u5206\u5b50\u306e\u5408\u6210\u3092\u89e6\u5a92\u3059\u308b\u9175\u7d20\u3067\u3059\u3002 DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u4e16\u4ee3\u304b\u3089\u4e16\u4ee3\u3078\u3068\u907a\u4f1d\u60c5\u5831\u3092\u5a18\u7d30\u80de\u306b\u6e21\u3059\u3053\u3068\u3092\u53ef\u80fd\u306b\u3059\u308bDNA\u8907\u88fd\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u307e\u3059\u3002<\/p>\n\n<figure class=\"wp-block-table\"><table class=\"has-subtle-pale-pink-background-color has-background\"><tbody><tr><td><a href=\"https:\/\/nebula.org\/whole-genome-sequencing\/\"><strong>DNA\u3092100\uff05\u30c7\u30b3\u30fc\u30c9\u3059\u308b\u3053\u3068\u306b\u8208\u5473\u304c\u3042\u308a\u307e\u3059\u304b\uff1f Nebula Genomics\u306f\u3001\u6700\u3082\u624b\u9803\u306a\u5168\u30b2\u30ce\u30e0\u30b7\u30fc\u30b1\u30f3\u30b9\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002 \u30b2\u30ce\u30e0\u30c7\u30fc\u30bf\u3078\u306e\u30d5\u30eb\u30a2\u30af\u30bb\u30b9\u3001\u6700\u65b0\u306e\u79d1\u5b66\u7684\u767a\u898b\u306b\u57fa\u3065\u304f\u6bce\u9031\u306e\u66f4\u65b0\u3001\u9ad8\u5ea6\u306a\u7956\u5148\u5206\u6790\u3001\u304a\u3088\u3073\u5f37\u529b\u306a\u30b2\u30ce\u30e0\u63a2\u7d22\u30c4\u30fc\u30eb\u3092\u4f7f\u7528\u3057\u3066\u3001\u4e00\u751f\u306e\u767a\u898b\u3092\u958b\u59cb\u3057\u307e\u3059\u3002 \u8a73\u7d30\u306b\u3064\u3044\u3066\u306f\u3001\u3053\u3053\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/strong><\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n<p><em>Christina Swords\u3001Ph.D\u3002\u304c\u7de8\u96c6<\/em><\/p>\n\n<h2 class=\"wp-block-heading\" id=\"h-biochemical-aspects-of-dna-polymerases\">DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u751f\u5316\u5b66\u7684\u5074\u9762<\/h2>\n\n<h3 class=\"wp-block-heading\" id=\"h-polymerase-activity\">\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u6d3b\u6027<\/h3>\n\n<p>\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u500b\u3005\u306e\u5206\u5b50\uff08\u30e2\u30ce\u30de\u30fc\uff09\u306e\u5316\u5b66\u7684\u7d50\u5408\u304c\u9396\uff08\u30dd\u30ea\u30de\u30fc\uff09\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002 DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u5834\u5408\u3001\u5f62\u6210\u3055\u308c\u308b\u30dd\u30ea\u30de\u30fc\u306f\u30c7\u30aa\u30ad\u30b7\u30ea\u30dc\u6838\u9178\uff08DNA\uff09\u3067\u3059\u3002 \u30e2\u30ce\u30de\u30fc\u306f\u30c7\u30aa\u30ad\u30b7\u30ea\u30dc\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3001\u3088\u308a\u6b63\u78ba\u306b\u306f\u30c7\u30aa\u30ad\u30b7\u30cc\u30af\u30ec\u30aa\u30b7\u30c9\u4e09\u30ea\u30f3\u9178\uff08dNTP\uff09\u3067\u3059\u3002 DNA\u4f9d\u5b58\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u5e38\u306b\u65e2\u5b58\u306eDNA\u4e00\u672c\u9396\u3092\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u3057\u3066\u4f7f\u7528\u3057\u3001\u305d\u306e\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u914d\u5217\u304c\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306b\u3088\u3063\u3066\u6c7a\u5b9a\u3055\u308c\u308b\u65b0\u3057\u3044\u76f8\u88dc\u9396\u3092\u5408\u6210\u3057\u307e\u3059\u3002 DNA\u914d\u5217\u306e\u3053\u306e\u4fdd\u5b58\u306f\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304cDNA\u306b\u30b3\u30fc\u30c9\u3055\u308c\u3066\u3044\u308b\u907a\u4f1d\u60c5\u5831\u3092\u30b3\u30d4\u30fc\u3059\u308b\u80fd\u529b\u306b\u3068\u3063\u3066\u6c7a\u5b9a\u7684\u3067\u3059\u3002 \u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u6b63\u3057\u3044\u30b3\u30d4\u30fc\u306f\u3001\u6c34\u7d20\u7d50\u5408\u306b\u3088\u3063\u3066\u5a92\u4ecb\u3055\u308c\u308b\u3001\u7d44\u307f\u8fbc\u307e\u308c\u305f\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u5869\u57fa\u3068DNA\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u5869\u57fa\u3068\u306e\u76f8\u88dc\u7684\u306a\u5869\u57fa\u5bfe\u5f62\u6210\u306b\u3088\u3063\u3066\u9054\u6210\u3055\u308c\u307e\u3059\u3002 \u65b0\u3057\u3044DNA\u9396\u306e\u5408\u6210\u306f\u30015 &#8216;\u304b\u30893&#8217;\u306e\u672b\u7aef\u307e\u3067\u884c\u308f\u308c\u307e\u3059\u3002 \u5316\u5b66\u7684\u306b\u306f\u3001dNTP\u306e\u03b1\u30ea\u30f3\u9178\u306b\u5bfe\u3059\u308bDNA\u9396\u306e\u672b\u7aef3&#8217;-\u30d2\u30c9\u30ed\u30ad\u30b7\u57fa\u306e\u6c42\u6838\u653b\u6483\u304c\u8d77\u3053\u308a\u3001\u30d4\u30ed\u30ea\u30f3\u9178\u304c\u653e\u51fa\u3055\u308c\u307e\u3059\u3002 \u3053\u306e\u30b9\u30c6\u30c3\u30d7\u306f\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306b\u3088\u3063\u3066\u89e6\u5a92\u3055\u308c\u307e\u3059\u3002<\/p>\n\n<p>RNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\uff08\u30a2\u30df\u30ce\u9178\u304b\u3089\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5408\u6210\u3059\u308b\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u308bRNA\u3092\u751f\u6210\u3059\u308b\uff09\u3068\u306f\u5bfe\u7167\u7684\u306b\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3067\u306e\u76f8\u88dc\u7684DNA\u9396\u306e\u5408\u6210\u306f\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u904a\u96e2\u306e3&#8242;-\u30d2\u30c9\u30ed\u30ad\u30b7\u672b\u7aef\u3092\u5229\u7528\u3067\u304d\u308b\u5834\u5408\u306b\u306e\u307f\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 \u6b21\u306b\u3001\u6700\u521d\u306e\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u304c\u3053\u306e\u672b\u7aef\u306b\u7d50\u5408\u3057\u307e\u3059\u3002 \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\uff08PCR\uff09\u3067\u306f\u3001\u9577\u3055\u304c\u7d0415\u301c20\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306eDNA\u4e00\u672c\u9396\uff08\u30d7\u30e9\u30a4\u30de\u30fc\uff09\u304c\u53cd\u5fdc\u306e\u958b\u59cb\u70b9\u3068\u3057\u3066\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002 \u9175\u7d20\u306f\u901a\u5e38\u3001\u88dc\u56e0\u5b50\u3068\u3057\u3066\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u3092\u5fc5\u8981\u3068\u3057\u307e\u3059\u3002<\/p>\n\n<p>\u30b8\u30a8\u30b9\u30c6\u30eb\u7d50\u5408\u306e\u5f62\u6210\u306e\u89e6\u5a92\u4f5c\u7528\u306f\u3001RNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u5bfe\u5fdc\u3059\u308b\u53cd\u5fdc\u3068\u6a5f\u80fd\u7684\u306b\u985e\u4f3c\u3057\u3066\u3044\u307e\u3059\u3002 \u3059\u3067\u306b\u5408\u6210\u3055\u308c\u305f\u30bb\u30af\u30b7\u30e7\u30f3\u306e\u6700\u5f8c\u306e\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3068\u8ffd\u52a0\u3055\u308c\u308b\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306f\u3001\u305d\u308c\u305e\u308c\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u30c9\u30e1\u30a4\u30f3\u306e\u89e6\u5a92\u4e2d\u5fc3\u306b\u3042\u308b2\u3064\u306e\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u306e1\u3064\u306b\u914d\u4f4d\u3057\u3066\u3044\u307e\u3059\u3002 \u8ffd\u52a0\u3055\u308c\u308b\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u6700\u521d\u306e\u30ea\u30f3\u9178\u57fa\u306f\u3001\u4e21\u65b9\u306e\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u306b\u914d\u4f4d\u3057\u3066\u3044\u307e\u3059\u3002 \u7a7a\u9593\u7684\u4f4d\u7f6e\u306b\u3088\u308a\u3001\u5148\u884c\u3059\u308b\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u30d2\u30c9\u30ed\u30ad\u30b7\u57fa\u304c\u3001\u4ed8\u52a0\u3055\u308c\u308b\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u30ea\u30f3\u9178\u57fa\u3092\u653b\u6483\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306b\u306a\u308b\u3002 \u305d\u306e\u904e\u7a0b\u3067\u3001\u30d4\u30ed\u30ea\u30f3\u9178\u6b8b\u57fa\u304c\u5206\u96e2\u3055\u308c\u307e\u3059\u3002<\/p>\n\n<figure class=\"wp-block-image size-large is-resized\"><img decoding=\"async\" src=\"https:\/\/nebula.org\/blog\/wp-content\/uploads\/2020\/08\/image-512x1024.png\" alt=\"DNA&#x30DD;&#x30EA;&#x30E1;&#x30E9;&#x30FC;&#x30BC;&#x306E;&#x6D3B;&#x6027;&#x90E8;&#x4F4D;&#x3067;&#x306E;&#x6A5F;&#x80FD;&#x3002;\" class=\"wp-image-2238\" width=\"256\" height=\"512\" srcset=\"https:\/\/nebula.org\/blog\/wp-content\/uploads\/2020\/08\/image-512x1024.png 512w, https:\/\/nebula.org\/blog\/wp-content\/uploads\/2020\/08\/image-150x300.png 150w, https:\/\/nebula.org\/blog\/wp-content\/uploads\/2020\/08\/image.png 462w\" sizes=\"(max-width: 256px) 100vw, 256px\" \/><figcaption>\u6838\u9178\u53cd\u5fdc\u304c\u89e6\u5a92\u3055\u308c\u308bDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u6d3b\u6027\u90e8\u4f4d\u3067\u306e\u30d7\u30ed\u30bb\u30b9\u3002<\/figcaption><\/figure>\n\n<h3 class=\"wp-block-heading\" id=\"h-exonuclease-activity\">\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027<\/h3>\n\n<p>\u591a\u304f\u306e\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u4ed6\u306e\u9175\u7d20\u6a5f\u80fd\u3082\u6301\u3063\u3066\u3044\u307e\u3059\u3002 \u4f4e\u6fc3\u5ea6\u306edNTP\u306e\u5b58\u5728\u4e0b\u3067\u306f\u3001\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u9664\u53bb\u306e\u305f\u3081\u306e3 &#8216;\u21925&#8217;\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u304c\u512a\u52e2\u3067\u3059\u3002 \u4e00\u90e8\u306e\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u30015 &#8216;\u21923&#8217;\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u3082\u6301\u3063\u3066\u3044\u307e\u3059\u3002 DNA\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u8aad\u307f\u53d6\u308a\u6642\u306b\u30a8\u30e9\u30fc\u304c\u767a\u751f\u3057\u306a\u3044\u3088\u3046\u306b\u3059\u308b\u305f\u3081\u306b\u3001\u3053\u306e\u6821\u6b63\u6a5f\u80fd\u304c\u3042\u308a\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u4e0d\u9069\u5207\u306a\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u633f\u5165\u3092\u691c\u51fa\u3057\u3001\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u306b\u3088\u3063\u3066DNA\u304b\u3089\u9664\u53bb\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 \u3053\u308c\u306b\u3088\u308a\u3001\u65b0\u3057\u3044\u9396\u304c\u5f62\u6210\u3055\u308c\u3066\u3044\u308b\u9593\u306b\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u9396\u3068\u3059\u3067\u306b\u30da\u30a2\u306b\u306a\u3063\u3066\u3044\u308b\u65e2\u5b58\u306eDNA\u307e\u305f\u306fRNA\u9396\u306e\u5206\u89e3\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3059\u3002 \u3053\u308c\u306b\u3088\u308a\u3001\u53e4\u3044\u30b9\u30c8\u30e9\u30f3\u30c9\u304c\u65b0\u3057\u3044\u30b9\u30c8\u30e9\u30f3\u30c9\u306b\u4ea4\u63db\u3055\u308c\u307e\u3059\u3002 \u3053\u306e\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u306f\u3001\u30cb\u30c3\u30af\u30c8\u30e9\u30f3\u30b9\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u306b\u3088\u3063\u3066\u5229\u7528\u3055\u308c\u307e\u3059\u3002<\/p>\n\n<h2 class=\"wp-block-heading\" id=\"h-different-dna-polymerases\">\u3055\u307e\u3056\u307e\u306aDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc<\/h2>\n\n<p>\u5927\u8178\u83cc\u306a\u3069\u306e\u7d30\u83cc\u306b\u306f\u30013\u3064\u306e\u7570\u306a\u308bDNA\u4f9d\u5b58\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u3042\u308a\u307e\u3059\u3002 \u305d\u308c\u3089\u306e1\u3064\u3067\u3042\u308bDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcI\uff08Pol I\uff09\u306f\u30011955\u5e74\u306bArthur Kornberg\u306b\u3088\u3063\u3066\u5206\u96e2\u3055\u308c\u3001\u3053\u308c\u307e\u3067\u306b\u767a\u898b\u3055\u308c\u305f\u6700\u521d\u306e\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3067\u3057\u305f\u3002 \u305f\u3060\u3057\u3001\u3053\u308c\u306f\u7d0420\u306e\u5408\u6210\u30b9\u30c6\u30c3\u30d7\u3057\u304b\u89e6\u5a92\u3057\u306a\u3044\u305f\u3081\u3001\u5927\u8178\u83cc\u3067\u306e\u8907\u88fd\u306b\u6700\u3082\u91cd\u8981\u306a\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3067\u306f\u3042\u308a\u307e\u305b\u3093\uff08\u3064\u307e\u308a\u3001\u51e6\u7406\u80fd\u529b\u304c\u4f4e\u3044\u3060\u3051\u3067\u3059\uff09\u3002 \u305f\u3060\u3057\u30015 &#8216;\u21923&#8217;\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u306e\u305f\u3081\u3001\u8907\u88fd\u4e2d\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u5206\u89e3\u306e\u539f\u56e0\u3068\u306a\u308a\u307e\u3059\u3002 \u5927\u8178\u83cc\u306e\u4ed6\u306e2\u3064\u306eDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3067\u3042\u308bDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcII\u3068DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcIII\u306f\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcI\u306e\u767a\u898b\u304b\u3089\u308f\u305a\u304b15\u5e74\u5f8c\u306b\u5206\u96e2\u3055\u308c\u307e\u3057\u305f\u3002\u305d\u306e\u5f8c\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcI\u907a\u4f1d\u5b50\u306b\u6b20\u9665\u304c\u3042\u308bE.coli\u5909\u7570\u4f53\u304c\u8907\u88fd\u3002 \u305f\u3060\u3057\u3001\u3053\u308c\u3089\u306e\u5909\u7570\u4f53\u306f\u7279\u306b\u7d2b\u5916\u7dda\u3084\u30a2\u30eb\u30ad\u30eb\u5316\u7269\u8cea\u306e\u5f71\u97ff\u3092\u53d7\u3051\u3084\u3059\u3044\u305f\u3081\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcI\u304c\u4e3b\u306b\u4fee\u5fa9\u4f5c\u696d\u3092\u884c\u3046\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002 \u5927\u8178\u83cc\u3067\u5b9f\u969b\u306e\u8907\u88fd\u3092\u884c\u3046\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcIII\u306f\u3001\u5408\u8a087\u3064\u306e\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u3067\u69cb\u6210\u3055\u308c\u3066\u304a\u308a\u3001\u7d30\u83cc\u7d30\u80de\u3042\u305f\u308a\u306e\u30b3\u30d4\u30fc\u6570\u306f\u3054\u304f\u308f\u305a\u304b\u3067\u3059\u3002<\/p>\n\n<p>\u30d2\u30c8DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3092\u542b\u3080\u771f\u6838\u751f\u7269\u306eDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u6b21\u306e\u30d5\u30a1\u30df\u30ea\u30fc\u306b\u5206\u985e\u3055\u308c\u307e\u3059\u3002<\/p>\n\n<ul class=\"wp-block-list\"><li>\u30d5\u30a1\u30df\u30ea\u30fcA\uff1aDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u03b3\u3001\u03b8\u3001\u304a\u3088\u3073\u03bd<\/li><li>\u30d5\u30a1\u30df\u30ea\u30fcB\uff1aDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u03b1\u3001\u03b4\u3001\u03b5\u304a\u3088\u3073\u03b6<\/li><li>\u30d5\u30a1\u30df\u30ea\u30fcX\uff1aDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u03b2\u3001\u03bb\u3001\u03c3\u304a\u3088\u3073\u03bc<\/li><li>\u30d5\u30a1\u30df\u30ea\u30fcY\uff1aDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u03b7\u3001\u03b9\u304a\u3088\u3073\u03ba<\/li><\/ul>\n\n<p>\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u03b3\u306f\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u3067\u306e\u307f\u767a\u751f\u3057\u307e\u3059\u3002<\/p>\n\n<p>\u54fa\u4e73\u985e\u3067\u306f\u3001\u03b1\u3001\u03b2\u3001\u03b3\u3001\u03b4\u3001\u03b5\u306e5\u7a2e\u985e\u3057\u304b\u767a\u751f\u3057\u307e\u305b\u3093\u3002 \u8907\u88fd\u306e\u6c7a\u5b9a\u8981\u56e0\u3067\u3042\u308b\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u03b4\u304a\u3088\u3073\u03b5\u306f\u3001\u9ad8\u3044\u51e6\u7406\u80fd\u529b\u3068\u6821\u6b63\u6a5f\u80fd\u3092\u7279\u5fb4\u3068\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002 \u5bfe\u7167\u7684\u306b\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u03b1\u304a\u3088\u3073\u03b2\u306f\u3001\u4f4e\u3044\u51e6\u7406\u80fd\u529b\u306e\u307f\u3092\u793a\u3057\u3001\u6821\u6b63\u6a5f\u80fd\u3092\u793a\u3055\u306a\u3044\u3002<\/p>\n\n<p>\u3055\u3089\u306b\u3001RNA\u3092\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u3057\u3066\u4f7f\u7528\u3057\u3001\u305d\u308c\u306bdNTP\u3092\u4ed8\u52a0\u3059\u308bRNA\u4f9d\u5b58\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u3042\u308a\u307e\u3059\u3002 \u3053\u308c\u3089\u306f\u9006\u8ee2\u5199\u9175\u7d20\u3068\u547c\u3070\u308c\u3001\u30c6\u30ed\u30e1\u30e9\u30fc\u30bc\u3082\u542b\u307e\u308c\u307e\u3059\u3002 \u552f\u4e00\u77e5\u3089\u308c\u3066\u3044\u308b\u72ec\u7acb\u3057\u305fDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u30bf\u30fc\u30df\u30ca\u30eb\u30c7\u30aa\u30ad\u30b7\u30ea\u30dc\u30cc\u30af\u30ec\u30aa\u30c1\u30b8\u30eb\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30e9\u30fc\u30bc\u3067\u3059\u3002<\/p>\n\n<p>\u53e4\u7d30\u83cc\u306b\u306f\u3001PCR\u306b\u3082\u4f7f\u7528\u3055\u308c\u308b\u6e29\u5ea6\u5b89\u5b9a\u578b\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n<h2 class=\"wp-block-heading\" id=\"h-biological-significance\">\u751f\u7269\u5b66\u7684\u610f\u7fa9<\/h2>\n\n<p>DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001DNA\u8907\u88fd\u306b\u3068\u3063\u3066\u6700\u3082\u91cd\u8981\u3067\u3059\u3002 \u305d\u308c\u3089\u306f\u3001DNA\u306e\u5f62\u3067\u907a\u4f1d\u5b50\u60c5\u5831\u306e\u5fe0\u5b9f\u306a\u30b3\u30d4\u30fc\u3092\u53ef\u80fd\u306b\u3057\u3001\u3057\u305f\u304c\u3063\u3066\u3001\u751f\u7269\u306e\u7e41\u6b96\u3068\u751f\u6b96\u306b\u304a\u3051\u308b\u6c7a\u5b9a\u7684\u306a\u30b9\u30c6\u30c3\u30d7\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002 \u9175\u7d20\u306f\u307e\u305f\u3001DNA\u4fee\u5fa9\u306b\u95a2\u9023\u3059\u308b\u30d7\u30ed\u30bb\u30b9\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u307e\u3059\u3002<\/p>\n\n<h2 class=\"wp-block-heading\" id=\"h-biotechnological-significance\">\u751f\u7269\u5de5\u5b66\u7684\u610f\u7fa9<\/h2>\n\n<p>\u7814\u7a76\u5ba4\u3067\u306f\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\u304a\u3088\u3073\u95a2\u9023\u3059\u308b\u65b9\u6cd5\uff08RT-PCR\u3001qPCR\u306a\u3069\uff09\u3001\u30cb\u30c3\u30af\u30c8\u30e9\u30f3\u30b9\u30ec\u30fc\u30b7\u30e7\u30f3\u3001\u30e9\u30f3\u30c0\u30e0\u30d7\u30e9\u30a4\u30df\u30f3\u30b0\u3001\u304a\u3088\u3073DNA\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u306b\u3088\u304f\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002 \u591a\u6570\u306e\u7570\u306a\u308b\u8010\u71b1\u6027\u30bf\u30a4\u30d7\uff08\u4f8b\uff1aTaq\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc<em>Thermus aquaticus<\/em> \uff09\u304c\u4f7f\u7528\u3055\u308c\u3001\u305d\u306e\u4e00\u90e8\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u5de5\u5b66\u306b\u3088\u3063\u3066\u5909\u66f4\u3055\u308c\u3066\u3044\u307e\u3059\u3002 \u9ad8\u6e29\u5b89\u5b9a\u6027\u306b\u52a0\u3048\u3066\u3001Pfu\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306a\u3069\u306e\u53e4\u98a8\u306a\u8d77\u6e90\u306e\u71b1\u5b89\u5b9a\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001PCR\u304c\u751f\u6210\u3055\u308c\u305fDNA\u306e\u5909\u5316\u3092\u5f15\u304d\u8d77\u3053\u3057\u3066\u306f\u306a\u3089\u306a\u3044\u305f\u3081\u3001\u8a3c\u62e0\u306e\u8aad\u307f\u53d6\u308a\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002 \u3055\u3089\u306b\u3001\u03c629DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306a\u3069\u306e\u9396\u7f6e\u63dbDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u5ba4\u6e29\u3067\u306e\u7b49\u6e29DNA\u5897\u5e45\u306e\u3055\u307e\u3056\u307e\u306a\u65b9\u6cd5\u3067\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002 \u4eca\u65e5\u4f7f\u7528\u3055\u308c\u3066\u3044\u308bDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u524d\u99c6\u4f53\u306f\u3001T4DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3067\u3057\u305f\u3002<\/p>\n\n<p>\u3053\u306e\u30d6\u30ed\u30b0\u6295\u7a3f\u306f\u6c17\u306b\u5165\u308a\u307e\u3057\u305f\u304b\uff1f \u767a\u898b\u3067\u304d\u308b<a href=\"https:\/\/nebula.org\/blog\/ja\/category\/%e7%90%86%e7%a7%91\/\">DNA\u3001\u907a\u4f1d\u5b66\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u306b\u95a2\u3059\u308b\u305d\u306e\u4ed6\u306e\u6295\u7a3f\u306f\u3053\u3061\u3089<\/a>\uff01<\/p>\n\n<p>\u6b21\u306e\u95a2\u9023\u30d6\u30ed\u30b0\u6295\u7a3f\u306b\u8208\u5473\u304c\u3042\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n\n<ul class=\"wp-block-list\"><li><a href=\"https:\/\/nebula.org\/blog\/ja\/dna-%e3%83%87%e3%82%aa%e3%82%ad%e3%82%b7%e3%83%aa%e3%83%9c%e6%a0%b8%e9%85%b8\/\">DNA\uff08\u30c7\u30aa\u30ad\u30b7\u30ea\u30dc\u6838\u9178\uff09<\/a><\/li><\/ul>\n\n<p><a href=\"https:\/\/nebula.org\/whole-genome-sequencing\/\">\u51fa\u767a\u3059\u308b\u524d\u306b\u3001\u5168\u30b2\u30ce\u30e0\u30b7\u30fc\u30b1\u30f3\u30b9\u3092\u30c1\u30a7\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\uff01<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u5b9a\u7fa9 DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u30c7\u30aa\u30ad\u30b7\u30ea\u30dc\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u304b\u3089\u306eD &hellip;<\/p>\n<p class=\"read-more\"> <a class=\"ast-button\" href=\"https:\/\/nebula.org\/blog\/ja\/dna-%e3%83%9d%e3%83%aa%e3%83%a1%e3%83%a9%e3%83%bc%e3%82%bc\/\"> <span class=\"screen-reader-text\">DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc-\u30b2\u30ce\u30e0\u306e\u5f37\u529b\u306a\u4f5c\u5bb6<\/span> Read More 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