{"id":13281,"date":"2021-01-31T22:14:00","date_gmt":"2021-02-01T03:14:00","guid":{"rendered":"https:\/\/nebula.org\/blog\/metodos-de-teste-de-introducao-ao-dna\/"},"modified":"2021-02-20T20:51:04","modified_gmt":"2021-02-21T01:51:04","slug":"metodos-de-teste-de-introducao-ao-dna","status":"publish","type":"post","link":"https:\/\/nebula.org\/blog\/pt-br\/metodos-de-teste-de-introducao-ao-dna\/","title":{"rendered":"Introdu\u00e7\u00e3o aos m\u00e9todos de teste de DNA"},"content":{"rendered":"\n

Editado por Christina Swords, Ph.D.<\/em><\/p>\n\n

O teste gen\u00e9tico nunca foi t\u00e3o f\u00e1cil. Os servi\u00e7os dom\u00e9sticos de teste de DNA continuam a crescer em popularidade como formas de aprender mais sobre sua ancestralidade e caracter\u00edsticas herdadas. No entanto, examinar todas as empresas e tipos de teste de DNA para encontrar o melhor teste de DNA pode ser opressor. Aqui, oferecemos uma breve vis\u00e3o geral dos diferentes tipos de testes de DNA. <\/p>\n\n

Tecnologias de teste de DNA<\/strong><\/h2>\n\n

DNA microarrays e Next Generation Sequencing (NGS) s\u00e3o duas tecnologias diferentes comumente usadas para testes gen\u00e9ticos. Ambos podem ser usados para ler informa\u00e7\u00f5es gen\u00e9ticas do DNA cromoss\u00f4mico e mitocondrial. No entanto, as tecnologias de microarrays de DNA e NGS funcionam de maneira muito diferente.<\/p>\n\n

Genotipagem baseada em DNA Microarray<\/h3>\n\n

Os microarranjos de DNA leem o DNA em um conjunto definido de posi\u00e7\u00f5es que variam entre as pessoas. Essas posi\u00e7\u00f5es s\u00e3o chamadas de polimorfismos de nucleot\u00eddeo \u00fanico (SNPs). A maioria dos servi\u00e7os de teste gen\u00e9tico, como 23andMe e AncestryDNA, usa a tecnologia de microarray de DNA para tra\u00e7ar o perfil (gen\u00f3tipo) de seu genoma em aproximadamente 500.000 posi\u00e7\u00f5es. Isso \u00e9 menos de 0,1% de todo o genoma humano. Embora a genotipagem baseada em microarray seja muito acess\u00edvel, ela perde muitas informa\u00e7\u00f5es importantes.<\/p>\n\n

Sequenciamento de pr\u00f3xima gera\u00e7\u00e3o<\/strong><\/h3>\n\n

O Sequenciamento de Pr\u00f3xima Gera\u00e7\u00e3o (NGS) \u00e9 uma tecnologia que permite a leitura de grandes quantidades de informa\u00e7\u00f5es gen\u00e9ticas com muita efici\u00eancia. NGS funciona lendo muitos pequenos trechos de DNA e, em seguida, juntando-os para determinar uma sequ\u00eancia cont\u00ednua (Figura 1). Em contraste com os microarranjos de DNA, o NGS pode ser usado para ler genomas inteiros em vez de apenas um pequeno n\u00famero de posi\u00e7\u00f5es. No entanto, embora o NGS produza muito mais dados do que a genotipagem baseada em microarray, ele tende a ser mais caro. Existem v\u00e1rios testes de DNA baseados em NGS que t\u00eam pre\u00e7os diferentes.<\/p>\n\n

\"Sequenciamento<\/figure>\n\n

Figura 1. Sequenciamento de pr\u00f3xima gera\u00e7\u00e3o (NGS). Imagem cortesia do National Human Genome Research Institute. <\/p>\n\n

Testes de DNA baseados em NGS<\/strong><\/h2>\n\n

Sequenciamento completo do exoma (WES)<\/strong><\/h3>\n\n

O Sequenciamento Completo do Exoma (WES) l\u00ea cerca de 1,5% do genoma humano que codifica prote\u00ednas – m\u00e1quinas moleculares que realizam a maioria das fun\u00e7\u00f5es celulares. Como o WES cobre apenas uma pequena por\u00e7\u00e3o do genoma humano, \u00e9 mais barato do que o WGS. No entanto, o WES perde variantes gen\u00e9ticas que est\u00e3o fora das regi\u00f5es codificadoras de prote\u00ednas e frequentemente t\u00eam fun\u00e7\u00f5es regulat\u00f3rias importantes. Al\u00e9m disso, o Sequenciamento completo do exoma ainda \u00e9 bastante caro quando comparado \u00e0 genotipagem baseada em microarray. Portanto, nossa resposta \u00e0 quest\u00e3o de escolher o Sequenciamento do Genoma Completo ou o Sequenciamento do Exoma Completo \u00e9 escolher o Sequenciamento do Genoma Completo.<\/p>\n\n

Sequenciamento do genoma completo (WGS)<\/strong><\/h3>\n\n

A tecnologia NGS pode ser usada para ler um genoma humano inteiro. Isso \u00e9 conhecido como Sequenciamento do Genoma Completo (WGS). Ele permite a descoberta imparcial e abrangente de variantes gen\u00e9ticas e produz uma vis\u00e3o mais profunda da composi\u00e7\u00e3o gen\u00e9tica pessoal. Al\u00e9m de ler todo o genoma, cada posi\u00e7\u00e3o \u00e9 frequentemente lida v\u00e1rias vezes para aumentar a precis\u00e3o do resultado. Por exemplo, o WGS \u00e9 normalmente feito com cobertura de 30x (profundidade), o que significa que em m\u00e9dia cada posi\u00e7\u00e3o no genoma \u00e9 lida 30 vezes. Isso torna o WGS muito mais caro do que a genotipagem baseada em microarray. <\/p>\n\n

Nebula Genomics<\/strong><\/h2>\n\n

Nossa miss\u00e3o na Nebula Genomics \u00e9 tornar o sequenciamento do genoma pessoal acess\u00edvel a todos. Em fevereiro de 2020, alcan\u00e7amos um marco importante ao trazer o custo de 30x Sequenciamento do genoma inteiro abaixo de US $ 300<\/a> . Isso aproxima o custo do sequenciamento do genoma pessoal do custo de testes gen\u00e9ticos muito menos abrangentes. <\/p>\n\n

Quer saber mais sobre como as tecnologias de teste de DNA s\u00e3o aplicadas? D\u00ea uma olhada em nosso introdu\u00e7\u00e3o ao teste de paternidade<\/a> !<\/p>\n","protected":false},"excerpt":{"rendered":"

Editado por Christina Swords, Ph.D. O teste gen\u00e9tico nunca foi t\u00e3o f\u00e1cil. Os servi\u00e7os dom\u00e9sticos de teste de DNA continuam a crescer em popularidade como formas de aprender mais sobre sua ancestralidade e caracter\u00edsticas herdadas. No entanto, examinar todas as …<\/p>\n

Introdu\u00e7\u00e3o aos m\u00e9todos de teste de DNA<\/span> Leia mais \u00bb<\/a><\/p>\n","protected":false},"author":18,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"site-sidebar-layout":"default","site-content-layout":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","_FSMCFIC_featured_image_caption":"","_FSMCFIC_featured_image_nocaption":"","_FSMCFIC_featured_image_hide":"","footnotes":""},"categories":[5002],"tags":[],"class_list":["post-13281","post","type-post","status-publish","format-standard","hentry","category-science-pt-br"],"yoast_head":"\nIntrodu\u00e7\u00e3o aos m\u00e9todos de teste de DNA - Nebula Genomics Blog<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nebula.org\/blog\/pt-br\/metodos-de-teste-de-introducao-ao-dna\/\" \/>\n<meta property=\"og:locale\" content=\"pt_BR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Introdu\u00e7\u00e3o aos m\u00e9todos de teste de DNA - Nebula Genomics Blog\" \/>\n<meta property=\"og:description\" content=\"Editado por Christina Swords, Ph.D. O teste gen\u00e9tico nunca foi t\u00e3o f\u00e1cil. 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