DayTwo review – Can this product help you get in shape?

DayTwo Review Summary

DayTwo is a gut microbiome test that provides personalized meal recommendations and consultation with a DayTwo dietician. Learn more in our DayTwo review!

Fiyin Ebemidayo

DayTwo app


DayTwo offers gut microbiome DNA testing to provide personalized diet recommendations and consultation with a registered dietitian. Its test and recommendations are available through organization health plans, making their price uncertain. Potential clients must contact the company for a quote.


7 Facts from our DayTwo Review

  1. Location: Brentwood, Tennessee
  2. Products: DayTwo’s microbiome testing kit. After completing the test, you get personalized meal recommendations on the DayTwo app and a one-on-one consultation with a dietician
  3. Reports: Detailed information about the diversity of the population of your gut microbiome, including food suggestions to increase the number of particular gut bacteria
  4. Privacy: The company claims to protect your data using administrative, technical, personnel, and physical measures. However, they do not mention the processes involved. Their privacy law is also based on the law applicable in Israel, where the company-owned labs operate
  5. Raw Data Access: You do not get access to raw data
  6. Cost: $499
  7. Complementary Products: Nebula Genomics (Whole Genome Sequencing with regularly updated reports (incl. diet), data exploration tools, and advanced ancestry reporting)

This review is written with the intent to be as unbiased as possible. However, it represents the opinion of an individual reviewer and is therefore subjective. Furthermore, at Nebula Genomics we seek to educate the public about the benefits of Whole Genome Sequencing. Information about our Whole Genome Sequencing DNA test is therefore incorporated into the review.

October 7, 2022

Pros and Cons


  • Easy-to-understand reports
  • Nutrition recommendations based on scientific research


  • Pricing is unknown
  • Only available to organizations as an offering on health plans
  • Not available on third-party sellers like Amazon


DayTwo is a personal nutrition-based microbiome company that uses the human gut biome to analyze different food recommendations and provide health solutions to consumers’ metabolic challenges. In brief, it is a personalized method going past diets and calorie counting. At this time, DayTwo is available through enrolled employer benefit programs and select health plans.

The DayTwo company started back in 2015. It is known for its accurate predictions and advice, which have provided excellent results based on groundbreaking scientific research. In addition, its app uses a mix of artificial intelligence and machine learning to build meal and snack smarter recommendations.

DayTwo homepage
DayTwo homepage

The company was co-founded by a lawyer named Lihi Segal, who currently leads its efforts. At first, its idea began with Professors Eran Segal and Eran Elinav from the Weizmann Institute of Science in Israel. Here, they published a five-year study on 1000 subjects to show how different human bodies respond to the same meals.

Their conclusion showed that the idea of a ‘perfect diet’ for all is vague, and everyone reacts differently, even if they have the same diet. Upon its completion, the research technology was licensed to DayTwo.

In a whitepaper by Mobi Health News, Eran Segal is quoted as saying: “DayTwo is the only evidence-based, actionable, microbiome platform in the market today.”

Company Mission and Partnerships

Lihi Segal, CEO and founder of DayTwo, said in a statement. “This deep and broad foundation, coupled with this financing, enables DayTwo to address the large and pressing clinical need to bring food-as-medicine to market for people with type 2 diabetes in the US.” Markedly, over 100 million people live with diabetes and pre-diabetes in the U.S.

DayTwo purchased the exclusive license for the ongoing research. DayTwo and Mayo Clinic then repeated it using 329 subjects. With this knowledge at its fingertips, it built a database and an app to provide different personalized nutrient recommendations.

Apart from these two companies, DayTwo also has partnerships with other health organizations like the Israel Diabetes Association, Joslin Diabetes Centre, and Janssen (a subsidiary of Johnson & Johnson). In addition, their collaboration with one of the world’s largest HMOs (Clalit) has helped push their product further in the gut microbiome testing world. 

If you are looking to sequence your gut, sequencing your genome is a smart move. Nebula Genomics offers 30X Whole Genome Sequencing to decode 100% of your DNA. Learn more about your ancestry, health, and traits with Whole Genome Sequencing! Click here to learn more!

Review of DayTwo Science: The Gut Microbiome and Blood Sugar Levels

Science has shown that the human gut has trillions of microbes (the microbiome), which play a role in how we metabolize specific food items and how our blood glucose levels vary after every meal.

The microbiome varies from person to person, and our blood glucose responses are different from each other even if we consume the same meal. This idea is what DayTwo uses to provide nutrition recommendations for its consumers all around the world. It also carried out a study using 800 people to verify this fact established by Prof. Segal and Prof. Elinav.

Plot of glucose levels with DayTwo versus a standard diet
Screenshot of example where glucose levels are lower and steadier with DayTwo advice than with a standard diet. Reference:

Here’s how the process goes: it conducts microbiome profiling using the full shotgun sequencing method from the stool sample you provide. Along with other details like your glycated hemoglobin reports and the surveys, DayTwo uses a combination of artificial intelligence and machine learning to provide unique analysis and meal recommendations that suit your gut microbiome.

DayTwo also suggets the type of food to eat to improve some deficient bacteria’s growth in your microbiome. The report shows different factors (such as butyrate, Vitamin B, and many more) needed in the body, the bacteria that augment the production of these factors, and the foods you should eat to promote the growth of these types of bacteria.


Through its partnership with the Joslin Diabetes Center, its product is specifically marketed to people with diabetes. Patients get a means of glycemic control that is way easier than the standard methods. They can monitor their blood sugar response without undergoing incessant nutrition plans, blood tests, and medications.

With DayTwo’s food-as-medicine plan, customers input different food types on the app and watch how it calculates their effect on their blood glycemic responses. Consultation with a dietician will also help determine appropriate meals even better. Its recommendations have also been found to be helpful with weight loss, liver diseases, and obesity.

However, DayTwo clearly states that its recommendations shouldn’t replace a health professional’s advice, and patients should always consult their doctors before using the product.

DayTwo Review

How to take a DayTwo test

After clicking on the ‘Get in Touch’ button, you are led to a page where you must decide between three options:: Business, Press, or Member. The DayTwo program is available for organizations to bring to their employees – individuals cannot purchase the kit.

The Member option will require some personal information like your name and email address. The company will send more details and get in touch through a call.

After you have all this set up, you get your gut microbiome testing kit within a week. The testing kit contains: 

  • ​​A toilet accessory.
  • A bio-specimen bag.
  • A mailer box.
  • A spatula.
  • A collector contained in a plastic tray.
DayTwo test pack
DayTwo test pack

After collecting your stool sample with the spatula and collector, put it in the bio-specimen bag, seal it, place the package in the mailer box, and send it back to the company’s labs. You should have scanned the barcode on the collector using the app before mailing it back to DayTwo.

The company will alert you through mail when your sample reaches its lab. You must also fill out a 10-minute questionnaire and submit blood reports, including your recent HbA1c levels. These reports help determine your current blood sugar levels.

The tests used by DayTwo
DayTwo approach to health

Within 21 days after mailing, you get your DayTwo results on the app, including meal recommendations and the report itself. You’ll also schedule a single session with one of DayTwo’s dietitians to help interpret your results.

Review of DayTwo Costs

As stated on its website, DayTwo is currently available through enrolled employer benefit programs and select health plans. Thus there is no pricing information available. For more information, including quotes, organizations can contact the company directly.

DayTwo Reports Review

After mailing your test kit, you get your gut microbiome profiling and personalized nutrition recommendations on the app within 21 days.


The report starts with a brief introduction to microbes and the gut microbiome. The introduction covers the gut microbiome’s function and how it’s related to health, nutrition, and immunity. It also tells you what to expect in your results. 


The report then moves on to the analysis of your results, starting with your bacterial composition, divided into four types: bacteriodetes, actinobacteria, proteobacteria, and firmicutes. It splits your share of these four bacterial types into percentages and compares them to its database population’s average average.

Sample of a microbiome composition in a DayTwo report
Sample of a microbiome composition in a DayTwo report

It then lists out your 50 most abundant bacterial types and their percentages and compares them to various percentages of its database population.

50 most abundant bacterial types listed in a DayTwo report
50 most abundant bacterial types listed in a DayTwo report

You can then see sections on your individual gut microbiome bacterial abundance ratios, important microbiome members (how your abundance compares with its database), and rare species (species in your gut that are notably different from the general population).

An interesting part of the report is the functional metabolite bacteria section. This section lists some essential metabolites in the body (such as butyrate) and the bacteria that augment their production. It then gives examples of foods that will help promote the growth of that particular bacterium.

DayTwo functional metabolites and suggestions
DayTwo functional metabolites and suggestions

The final sections detail analysis of ecological parameters (richness, evenness, and diversity) and probiotic bacteria. They also include information on DNA sequencing technology and references.

The app

As detailed and simple as the report is, the app is even easier to use. The scoring system rates thousands of different foods and combinations based on your biometrics, gut microbiome analysis, lifestyle factors, and questionnaire, yielding a unique nutrition profile that ensures a healthy blood-sugar balance for long-term health. You can also ask for a minimalized list of “Your Search Results” when building a meal.

This way, all you need to do is input a particular meal regime, and the machine learning algorithm calculates how it affects your blood sugar level. It also creates food recommendations specific to your body, like Top Meals for breakfast, lunch, and dinner.

Overall, DayTwo provides excellent reports and helps you understand them better, thanks to its dietician session. A sample report can be found through an internet search.

DayTwo Privacy Review

The privacy policy is quite explanatory, although it could have better explained its security measures.

In using the website, you are giving DayTwo permission to use your data to provide its services. But this information can also be used to improve such services for all users. It is important to note that you also automatically grant permission to capture video recordings of you, which will occur during telehealth and group sessions.

Sending your stool sample to DayTwo is agreeing that lab personnel will review and analyze it either in the US or Israel labs. Where this takes place is at the company’s sole discretion. This information will be treated as protected health information but can still be connected to you.

DayTwo claims it uses personal data for its services only. While it might share your data with third-party websites like cloud storage and data processing solutions, it argues these services uphold privacy practices and help deliver its services only.

In terms of security, it claims to use administrative, technical, personnel, and physical measures to protect your data. However, it fell short of telling more about its protection measures. 

Other DayTwo Reviews

Due to the limited availability of its products on other platforms, there are not many reviews available. However, DNA Testing Choice users provide three reviews. While two users gave good reviews and five-star ratings about the product, the last one complained about not getting a report after months of submitting a sample and gave a one-star rating. This resulted in a cumulative rating of 4.5 stars.

There are several testimonials on the DayTwo website, with quantitative values demonstrating the effectiveness of the product.

Testimonials available on the company website
DayTwo testimonials available on its website

DayTwo in the News

Also in 2022, DayTwo compiled its National Book of Business Outcomes. This document reflects participants who completed 12 consecutive months of the DayTwo program.

Nebula Genomics

Knowing your genetic predispositions to high blood sugar levels could help determine if you should use products like the DayTwo’s Gut Microbiome test. You can learn more about genetic variants related to high blood sugar levels in the Nebula Research Library.

At Nebula Genomics, our job is to give you the best Whole Genome Sequencing technique money can buy. Our test proudly stands above other kits. This is because we analyze over 6,000,000,000 positions on your DNA. We’re experts in this field, and our expertise is reflected in the detailed report we give to every consumer.

We also offer genetic testing, which provides deep ancestry reporting with a full Y chromosome and mtDNA sequencing. Our collaborations make it easier for us to map out your family tree. This way, you can satisfy your curiosity about your origins. 

We also give you the option to upload raw DNA data to our website for additional DNA analysis. 

Unlike DayTwo, our privacy policies are very transparent. We use privacy computing and blockchain-type encryption on our website to ensure no one has complete access to your data except you.

DayTwo vs. Nebula Genomics

ViomeDayTwoNebula Genomics
Testing MethodStool StoolSaliva and Whole genome sequencing at 30x coverage
ReportingGut microbes present, proprietary “health scores”Microbiome diversity, effect on food metabolism/blood sugar levels, diets based on this informationComprehensive genetic predispositions
Ancestry reportingNoNoDeep ancestry reporting with full Y chromosome and mtDNA sequencing
Data upload optionsNoNoYes (learn more)
Focus on privacyNoNoYes (learn more)
Tools to explore dataNoNoYes
Cost$149 / $349N/A$0, $99, $299

Other companies

You should also check out these other companies that offer DNA testing for gut microbiome and diet recommendations:

  • Atlas Biomed (microbiome and nutrition recommendations)
  • Biohm (gut health, including bacteria and fungi)
  • Biomesight (gut testing and recommendations)
  • Ixcela (blood test for gut health and personalized recommendations)
  • Kean Health (gut health, guidance, plus a gene test with 100+ traits)
  • OlaWell (recommendations to avoid food intolerances)
  • Onegevity (gut health and customized recommendations)
  • Psomagen (gut health and gene test)
  • Rootine (personalized supplement and vitamins based on DNA test)
  • Sun Genomics (gut microbiome testing and personalized probiotics for adults, children, and infants)
  • Ombre (formally Thryve) (personalized probiotics)
  • Verisana (hormone, gut, and STD tests plus many more)
  • Viome (gut health and microbiome testing)

Nutritional supplements and weight loss programs that may also be good to explore:

  • Goli Nutrition (apple cider vinegar gummies)
  • Hydroxycut (weight loss supplements)
  • InsideTracker (biomarker health tests and optional DNA test)
  • Resurge (deep sleep supplement for weight loss)
  • Rootine (personalized supplement and vitamins based on DNA test)
  • Noom (subscription-based weight loss programs)
  • Nutrisystem (customized weight loss programs)
  • Yoga Burn (instructor-led weight loss yoga)

Did you find our DayTwo review helpful? You can read more on our blog and check out our complete guide to the best DNA test kit and other home tests.

You may also be interested in virtual care such as that offered by Teladoc and Lemonaid Health!

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